Exercise PMF4
Topic
To investigate how PMF mixtures are reported
Experimental
MALDI-TOF data from a 2D gel spot. Protein(s) are from yeast.
Enzyme trypsin, state of cysteine unimportant, mass tolerance 100 ppm. The data file contains a list of MH+ values
Data
Right-click this link:
PMF4.txt,
and choose Save to file
Tips
- Try searching SwissProt and NCBInr, with and without a taxonomy filter
Questions
- What are the effects of database size?
- How many components are you confident are present?
- Does the search engine report a choice of mixtures? If so, what does this mean?
Hints |
Aldente results |
Mascot results |
MS-Fit results |
Profound results |
Answers
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