MS-Fit Search Results

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Fraction-Spot-Run ID: 1-1-1
MS-Fit search selects 62 entries (results displayed for top 5 matches).

[–] Results Summary

Protein Hit Number	MOWSE Score	# pep # mat % mat 32 pks	% Cov	% TIC	Mean Err ppm	Data Tol ppm	# Hom Prot	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	51941	9/8/25	17.4	25.0	32.6	21.5	No	252190	65783/5.5	P33755	YEAST	Nuclear protein localization protein 4
2	5114	11/10/31	15.7	31.2	37.5	12.0	No	52417	91997/4.8	P25694	YEAST	Cell division control protein 48
3	210	4/4/12	12.1	12.5	11.9	59.3	No	510429	35912/6.9	P40101	YEAST	Uncharacterized protein YER186C
4	209	4/4/12	6.8	12.5	24.9	136	No	410015	101083/9.2	P40317	YEAST	Protein SOK1
5	200	4/4/12	6.6	12.5	-10.7	116	No	285140	99356/5.9	P14242	YEAST	DNA mismatch repair protein PMS1

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[+] Detailed Results

1. 8/32 matches (25%).
Acc. #: P33755 Species: YEAST Name: Nuclear protein localization protein 4
Index: 252190 MW: 65783 Da pI: 5.5

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
755.3741	755.3505	100.0	31.3	254	259	0	(R)YTGMQR(F)
799.4375	799.4169	100.0	25.7	7	13	1	(R)SKNGTHR(V)
799.4375	799.4308	100.0	8.33	125	131	0	(K)EDGLIPR(Q)
1019.5351	1019.4939	100.0	40.5	313	321	0	(R)QAQEMGLSR(I)
1384.7111	1384.6644	100.0	33.7	481	492	0	(K)FVSSTGFPWSNR(Q)
1505.8222	1505.7747	100.0	31.5	242	253	0	(K)SEIINEFIQAWR(Y)
1685.9182	1685.8567	100.0	36.5	346	360	0	(K)DSFFLSSLEVIMAAR(H)
1797.9055	1797.8290	100.0	42.6	184	199	0	(K)ENGSSYISPLSEPDFR(I)
1954.0545	1953.9705	100.0	43.0	507	523	0	(K)YLFNVASSGDFNLLHEK(I)

Num Unmatched Masses: 24
Search for disulfide linked peptides. Do a non-specific cleavage search. Search for another component.

The matched peptides cover 17.4% (101/580AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 252190

2. 10/32 matches (31%).
Acc. #: P25694 Species: YEAST Name: Cell division control protein 48
Index: 52417 MW: 91997 Da pI: 4.8

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
797.4272	797.4053	100.0	27.5	370	375	1	(R)FGRFDR(E)
851.5758	851.5461	100.0	34.8	242	249	0	(K)AIGIKPPR(G)
957.5615	957.5265	100.0	36.6	158	165	1	(R)KGDHFVVR(G)
1027.6680	1027.6259	100.0	41.0	664	672	0	(R)LSILNAQLR(K)
1246.7006	1246.6539	100.0	37.5	464	475	0	(R)FALGNSNPSALR(E)
1246.7006	1246.6638	100.0	29.6	18	28	1	(R)EEDKTATAILR(R)
1302.7299	1302.6801	100.0	38.2	707	717	1	(K)YAIKDSIEAHR(Q)
1737.0195	1736.9516	100.0	39.1	228	241	1	(R)EMVELPLRHPQLFK(A)
1756.0038	1755.9276	100.0	43.4	649	663	0	(R)LDQLIYVPLPDENAR(L)
1853.1038	1853.0127	100.0	49.2	376	392	1	(R)EVDIGIPDATGRLEVLR(I)
2107.2376	2107.1618	100.0	36.0	349	368	0	(R)SNVVVIAATNRPNSIDPALR(R)

Num Unmatched Masses: 22
Search for disulfide linked peptides. Do a non-specific cleavage search. Search for another component.

The matched peptides cover 15.7% (131/835AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 52417

3. 4/32 matches (12%).
Acc. #: P40101 Species: YEAST Name: Uncharacterized protein YER186C
Index: 510429 MW: 35912 Da pI: 6.9

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
1302.7299	1302.7562	100.0	-20.2	168	178	1	(R)ILTLVRCSEIR(S)
1400.7087	1400.6416	100.0	47.9	1	11	0	(-)MLWHPDGYEPR(V)
1797.9055	1797.9098	100.0	-2.39	96	109	0	(R)DYVGFLFYQYEILK(N)
1954.0545	1954.0109	100.0	22.3	95	109	1	(R)RDYVGFLFYQYEILK(N)

Num Unmatched Masses: 28
Search for disulfide linked peptides. Do a non-specific cleavage search. Search for another component.

The matched peptides cover 12.1% (37/306AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 510429

4. 4/32 matches (12%).
Acc. #: P40317 Species: YEAST Name: Protein SOK1
Index: 410015 MW: 101083 Da pI: 9.2

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
755.3741	755.4298	100.0	-73.7	175	181	0	(K)VDSIPPK(N)
1853.1038	1852.9552	100.0	80.2	200	217	0	(K)QPPSASAPQLPPATEPHK(E)
1927.0255	1926.9556	100.0	36.3	182	199	0	(K)NIYTNINNPSPSPPPSSK(Q)
2107.2376	2107.1182	100.0	56.7	765	782	1	(K)QTQPLSEVYGVLENRVFK(S)

Num Unmatched Masses: 28
Search for disulfide linked peptides. Do a non-specific cleavage search. Search for another component.

The matched peptides cover 6.8% (61/901AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 410015

5. 4/32 matches (12%).
Acc. #: P14242 Species: YEAST Name: DNA mismatch repair protein PMS1
Index: 285140 MW: 99356 Da pI: 5.9

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
1019.5351	1019.5884	100.0	-52.3	81	89	1	(K)IAKFQDVAK(V)
1719.9317	1719.8595	100.0	42.0	1	14	0	(-)MTQIHQINDIDVHR(I)
1780.8845	1781.0068	100.0	-68.7	142	157	0	(K)GTTVLVSQLFHNLPVR(Q)
2146.1804	2146.1026	100.0	36.2	30	48	0	(K)ELVDNSIDANANQIEIIFK(D)

Num Unmatched Masses: 28
Search for disulfide linked peptides. Do a non-specific cleavage search. Search for another component.

The matched peptides cover 6.6% (58/873AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 285140