Exercise PMF2
Topic
A more difficult PMF search. Learn to expect the unexpected!
Experimental
A protein from a 2D gel spot (not alkylated) was digested with trypsin and analysed on a reflector MALDI-TOF instrument.
The instrument is supposed to be capable of 25ppm accuracy, but it has not been used for a very long
time and the person who ran the spectrum is a novice. The gel was run as part of a study of baker's yeast,
(Saccharomyces Cerevisiae). The data file contains a list of MH+ values
Data
Right-click this link:
PMF2.txt,
and choose Save to file
Tips
- The protein sequence is in SwissProt
- A strong match
- Modification state of Cysteine is unimportant
Questions
- What can we learn from this result?
- What advice would you give to the person who ran the spectrum?
Hints |
Aldente results |
Mascot results |
MS-Fit results |
Profound results |
Answers
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