MS-Fit Search Results

Doing Pre Search. Doing Protein MW search. Search Processing 22% completed. 2 sec elapsed. 7 sec remaining. Search Processing 57% completed. 4 sec elapsed. 3 sec remaining. Search Processing 96% completed. 6 sec elapsed. 0 sec remaining. Search completed. 6 sec elapsed. 0 sec remaining. Calculating detailed results please wait. This can take some time.

[+] Parameters

Database searched: SwissProt.2011.01.11
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Minimum Matches: 4
Sort Type: Score Sort
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4
Report Homologous Proteins: Interesting

[+] Pre Search Results

Number of entries in the database: 524420
Molecular weight search (1000 - 125000 Da) selects 512308 entries.
Full pI range: 524420 entries.
Combined molecular weight and pI searches select 512308 entries.
Pre searches select 512308 entries.

Fraction-Spot-Run ID: 1-1-1
MS-Fit search selects 973 entries (results displayed for top 5 matches).

[–] Results Summary

Protein Hit Number	MOWSE Score	# pep # mat % mat 18 pks	% Cov	% TIC	Mean Err ppm	Data Tol ppm	# Hom Prot	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	2.99e+8	15/15/83	30.5	83.3	200	22.8	No	186760	58828/5.1	P13645	HUMAN	Keratin, type I cytoskeletal 10
2	10545	7/7/39	10.8	38.9	111	287	No	67716	114198/8.7	Q0VF58	MOUSE	Collagen alpha-1(XIX) chain
3	2591	4/4/22	10.0	22.2	171	71.8	No	59397	79037/6.3	Q6DCT2	XENLA	UPF0668 protein C10orf76 homolog
4	2274	5/5/28	19.3	27.8	108	333	No	26884	46054/5.9	Q9X2A1	THEMA	Argininosuccinate synthase

186760	x	.	x	x	x	x	x	x	x	x	.	x	x	.	x	x	x	x
67716	.	.	.	.	.	x	.	.	x	.	.	x	x	.	x	.	x	x
59397	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	x	x
26884	x	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	x	x

[+] Detailed Results

1. 15/18 matches (83%).
Acc. #: P13645 Species: HUMAN Name: Keratin, type I cytoskeletal 10
Index: 186760 MW: 58828 Da pI: 5.1

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
809.5819	809.4403	100.0	175	157	163	0	(R)LASYLDK(V)
993.7087	993.5000	100.0	210	238	245	0	(K)YENEVALR(Q)
1003.7507	1003.5531	100.0	197	363	370	1	(K)SEITELRR(N)
1090.7481	1090.5310	100.0	199	148	156	0	(K)VTMQNLNDR(L)
1165.8431	1165.5848	100.0	222	442	450	0	(R)LENEIQTYR(S)
1234.9169	1234.6790	100.0	193	236	245	1	(R)LKYENEVALR(Q)
1357.9915	1357.7183	100.0	201	246	257	1	(R)QSVEADINGLRR(V)
1365.9131	1365.6393	100.0	200	323	333	0	(R)SQYEQLAEQNR(K)
1435.0470	1434.7700	100.0	193	440	450	1	(K)IRLENEIQTYR(S)
1494.0287	1493.7343	100.0	197	323	334	1	(R)SQYEQLAEQNRK(D)
1708.1516	1707.7721	100.0	222	41	59	0	(K)GSLGGGFSSGGFSGGSFSR(G)
1995.3290	1994.9308	100.0	200	16	40	1	(R)SGGGGGGGGCGGGGGVSSLRISSSK(G)
1997.3694	1996.9710	100.0	200	346	362	0	(K)ELTTEIDNNIEQISSYK(S)
2367.7335	2367.2627	100.0	199	208	228	0	(K)NQILNLTTDNANILLQIDNAR(L)
2872.9645	2872.3928	100.0	199	296	322	0	(R)NVSTGDVNVEMNAAPGVDLTQLLNNMR(S)

Num Unmatched Masses: 3

Search for disulfide linked peptides.

Do a non-specific cleavage search.

Search for another component.

The matched peptides cover 30.5% (178/584AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 186760

2. 10/18 matches (55%).
Acc. #: Q6EIZ0 Species: CANFA Name: Keratin, type I cytoskeletal 10
Index: 186759 MW: 57711 Da pI: 5.1

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
809.5819	809.4403	100.0	175	147	153	0	(R)LASYLDK(V)
993.7087	993.5000	100.0	210	228	235	0	(K)YENEVALR(Q)
1003.7507	1003.5531	100.0	197	353	360	1	(K)SEITELRR(T)
1090.7481	1090.5310	100.0	199	138	146	0	(K)VTMQNLNDR(L)
1165.8431	1165.5848	100.0	222	432	440	0	(R)LENEIQTYR(S)
1234.9169	1234.6790	100.0	193	226	235	1	(R)LKYENEVALR(Q)
1357.9915	1357.7183	100.0	201	236	247	1	(R)QSVEADINGLRR(V)
1435.0470	1434.7700	100.0	193	430	440	1	(K)IRLENEIQTYR(S)
1708.1516	1707.7721	100.0	222	35	53	0	(K)GSIGGGFSSGGFSGGSFSR(G)
2367.7335	2367.2627	100.0	199	198	218	0	(K)NQILNLTTDNANILLQIDNAR(L)

Num Unmatched Masses: 8

Search for disulfide linked peptides.

Do a non-specific cleavage search.

Search for another component.

The matched peptides cover 17.1% (97/568AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 186759

3. 7/18 matches (38%).
Acc. #: Q0VF58 Species: MOUSE Name: Collagen alpha-1(XIX) chain
Index: 67716 MW: 114198 Da pI: 8.7

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
1165.8431	1165.6575	100.0	159	489	500	0	(R)GEPGLLGPQGIK(G)
1365.9131	1365.7022	100.0	154	723	736	1	(K)GDVGPRGPPGFPGR(E)
1494.0287	1493.8071	100.0	148	486	500	1	(K)GDRGEPGLLGPQGIK(G)
1708.1516	1707.8813	100.0	158	456	473	1	(K)GDTGLPGFPGSVGPKGHK(G)
1995.3290	1995.0406	100.0	145	603	624	0	(R)GLPGLHGSPGDTGPPGVGIPGR(T)
2367.7335	2367.2051	100.0	223	571	596	0	(K)GDAGPPGISLPGKPGLDGNPGSPGPR(G)
2872.9645	2873.5641	100.0	-209	1028	1055	1	(R)MAVFLSQLKLPAAMLSAQAHGRPGPPGK(D)

Num Unmatched Masses: 11

Search for disulfide linked peptides.

Do a non-specific cleavage search.

Search for another component.

The matched peptides cover 10.8% (123/1136AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 67716

4. 4/18 matches (22%).
Acc. #: Q6DCT2 Species: XENLA Name: UPF0668 protein C10orf76 homolog
Index: 59397 MW: 79037 Da pI: 6.3

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Sequence
1357.9915	1357.7072	100.0	209	25	35	(K)VVLMYDEIFTK(E)
1365.9131	1365.7446	100.0	123	506	517	(K)FLMSNETVLLAK(H)
2367.7335	2367.3031	100.0	182	290	310	(K)ISVQTNEAILLALYEAVHLNR(N)
2872.9645	2872.4778	100.0	169	437	461	(K)NIPCRPLVCAVLDLMVEFIVTHMMK(D)

Num Unmatched Masses: 14

Search for disulfide linked peptides.

Do a non-specific cleavage search.

Search for another component.

The matched peptides cover 10.0% (69/689AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 59397

5. 5/18 matches (27%).
Acc. #: Q9X2A1 Species: THEMA Name: Argininosuccinate synthase
Index: 26884 MW: 46054 Da pI: 5.9

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
809.5819	809.4767	100.0	130	347	353	0	(K)VTVSIYK(G)
1435.0470	1434.7773	100.0	188	264	275	1	(R)LDMVENRFIGIK(S)
1494.0287	1493.7999	100.0	153	126	138	0	(R)FELTYAALNPNLK(V)
2367.7335	2368.1555	100.0	-178	238	258	1	(K)DGTEKTDPLELFEYLNEVGAK(N)
2872.9645	2872.2506	100.0	249	362	387	0	(R)YSPYSLYNPELSSMDVEGGFDATDSK(G)

Num Unmatched Masses: 13

Search for disulfide linked peptides.

Do a non-specific cleavage search.

Search for another component.

The matched peptides cover 19.3% (79/409AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 26884