Exercise PMF1
Topic
A PMF that gives a very strong match. Treat it as a standard and use it to find optimum settings for the search parameters
Experimental
A protein mixture (not alkylated) was separated on a 2D gel. A spot was extracted and digested with trypsin.
The digest was analysed on a reflector MALDI-TOF instrument.
The data file contains a list of MH+ values
Data
Right-click this link:
PMF1.txt,
and choose Save to file
Tips
- The protein sequence is in SwissProt
- A very strong match
Questions
- What is a suitable mass tolerance?
- What is the optimum setting for missed cleavages?
- Is there evidence for Met-Ox?
Hints |
Aldente results |
Mascot results |
MS-Fit results |
Profound results |
Answers
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