MS-Fit Search Results

Doing Pre Search. Doing Protein MW search. Search Processing 5% completed. 2 sec elapsed. 38 sec remaining. Search Processing 15% completed. 4 sec elapsed. 22 sec remaining. Search Processing 23% completed. 6 sec elapsed. 20 sec remaining. Search Processing 32% completed. 8 sec elapsed. 17 sec remaining. Search Processing 41% completed. 10 sec elapsed. 14 sec remaining. Search Processing 48% completed. 12 sec elapsed. 13 sec remaining. Search Processing 57% completed. 14 sec elapsed. 10 sec remaining. Search Processing 68% completed. 16 sec elapsed. 7 sec remaining. Search Processing 78% completed. 18 sec elapsed. 5 sec remaining. Search Processing 83% completed. 20 sec elapsed. 4 sec remaining. Search Processing 91% completed. 22 sec elapsed. 2 sec remaining. Search completed. 24 sec elapsed. 0 sec remaining. Calculating detailed results please wait. This can take some time.

[+] Parameters

Database searched: SwissProt.2011.01.11
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Oxidation of M |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4
Report Homologous Proteins: Interesting

[+] Pre Search Results

Number of entries in the database: 524420
Molecular weight search (1000 - 125000 Da) selects 512308 entries.
Full pI range: 524420 entries.
Combined molecular weight and pI searches select 512308 entries.
Pre searches select 512308 entries.

Fraction-Spot-Run ID: 1-1-1
MS-Fit search selects 67411 entries (results displayed for top 5 matches).

[–] Results Summary

Protein Hit Number	MOWSE Score	# pep # mat % mat 135 pks	% Cov	% TIC	Mean Err ppm	Data Tol ppm	# Hom Prot	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	2.00e+15	33/31/23	53.6	23.0	-0.938	31.5	No	172915	52949/6.8	P07099	HUMAN	Epoxide hydrolase 1
2	2.29e+7	18/14/10	20.6	10.4	3.75	54.8	No	523380	124290/9.0	Q92618	HUMAN	Zinc finger protein 516
3	1.18e+7	13/13/10	15.9	9.6	-3.42	42.3	No	47130	119164/5.3	A2BJL3	HYPBU	Carbamoyl-phosphate synthase large chain
4	9.93e+6	14/13/10	29.2	9.6	2.94	61.5	No	228430	64670/9.1	Q67C40	ORYSJ	Mitogen-activated protein kinase 7
5	7.58e+6	12/12/9	15.2	8.9	-2.96	48.1	No	315543	114477/7.7	Q9SLZ4	PEA	Retinoblastoma-related protein 1

172915	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	x	.	.	x	x	x	.	x	x	x	x	.	.	x	x	.	.	x	x	.	x	.	.	x	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	x	.	.	.	.	.	x	.	.	.	x	.	.	.	.	x	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.
523380	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	x	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.
47130	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
228430	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	x	.	.	.	.	x	x	x	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.
315543	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	x	.	x	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.

[+] Detailed Results

1. 31/135 matches (22%).
Acc. #: P07099 Species: HUMAN Name: Epoxide hydrolase 1
Index: 172915 MW: 52949 Da pI: 6.8

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Modifications	Start	End	Missed Cleavages	Sequence
823.4300	823.4461	100.0	-19.6		93	98	0	(K)VISYWR(N)
835.4600	835.4672	100.0	-8.65		271	277	0	(R)FLGLTER(D)
838.3700	838.3730	100.0	-3.57		99	104	0	(R)NEFDWK(K)
863.4900	863.4985	100.0	-9.88		448	454	0	(K)FLSVLER(Q)
871.4900	871.4996	100.0	-11.0		106	112	0	(K)QVEILNR(Y)
915.4700	915.4757	100.0	-6.21		289	295	0	(K)VFYSLMR(E)
922.4900	922.4741	100.0	17.2		198	206	0	(K)GFNSVATAR(I)
931.4800	931.4706	100.0	10.1	1Oxidation	289	295	0	(K)VFYSLMR(E)
951.5300	951.5411	100.0	-11.6		92	98	1	(K)KVISYWR(N)
966.4800	966.4680	100.0	12.5		99	105	1	(R)NEFDWKK(Q)
991.5700	991.5571	100.0	13.0		448	455	1	(K)FLSVLERQ(-)
991.5700	991.5935	100.0	-23.7		447	454	1	(R)KFLSVLER(Q)
1009.6100	1009.6292	100.0	-19.0		160	168	0	(K)IIPLLTDPK(N)
1050.5900	1050.5691	100.0	19.9		197	206	1	(K)KGFNSVATAR(I)
1051.5300	1051.5055	100.0	23.3		339	347	0	(R)YLEDGGLER(K)
1075.6000	1075.6034	100.0	-3.15		278	286	0	(R)DVELLYPVK(E)
1106.5700	1106.5477	100.0	20.2		44	52	0	(R)EDDSIRPFK(V)
1131.6000	1131.5867	100.0	11.8		420	428	0	(K)LISYSYMVR(G)
1147.5900	1147.5816	100.0	7.31	1Oxidation	420	428	0	(K)LISYSYMVR(G)
1288.6100	1288.5957	100.0	11.1		329	338	0	(K)FSTWTNTEFR(Y)
1307.5700	1307.6049	100.0	-26.7	1Oxidation	376	386	0	(K)ENLGQGWMTQK(H)
1729.7900	1729.8075	100.0	-10.1	1Oxidation	376	389	1	(K)ENLGQGWMTQKHER(M)
1729.7900	1729.8367	100.0	-27.0		373	386	1	(R)FYKENLGQGWMTQK(H)
1745.7900	1745.8316	100.0	-23.8	1Oxidation	373	386	1	(R)FYKENLGQGWMTQK(H)
1842.8500	1842.8657	100.0	-8.52		24	39	0	(K)EETLPLEDGWWGPGTR(S)
1892.0100	1892.0528	100.0	-22.6		271	286	1	(R)FLGLTERDVELLYPVK(E)
1999.9900	1999.9872	100.0	1.39		429	446	0	(R)GGHFAAFEEPELLAQDIR(K)
2085.9900	2085.9876	100.0	1.15		22	39	1	(R)DKEETLPLEDGWWGPGTR(S)
2128.0800	2128.0822	100.0	-1.02		429	447	1	(R)GGHFAAFEEPELLAQDIRK(F)
2279.1900	2279.1747	100.0	6.72		392	411	0	(K)VYVPTGFSAFPFELLHTPEK(W)
2527.4200	2527.4065	100.0	5.35	1Oxidation	245	267	0	(K)GLHLNMALVLSNFSTLTLLLGQR(F)
2795.4000	2795.3231	100.0	27.5		44	66	1	(R)EDDSIRPFKVETSDEEIHDLHQR(I)
2902.5500	2902.5019	100.0	16.6		348	372	1	(R)KFSLDDLLTNVMLYWTTGTIISSQR(F)

Num Unmatched Masses: 104

Search for disulfide linked peptides.

Do a non-specific cleavage search.

Search for another component.

The matched peptides cover 53.6% (244/455AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 172915

2. 14/135 matches (10%).
Acc. #: Q92618 Species: HUMAN Name: Zinc finger protein 516
Index: 523380 MW: 124290 Da pI: 9.0

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
827.4500	827.4118	100.0	46.1	717	724	1	(K)EHSGGGKR(A)
827.4500	827.4233	100.0	32.3	675	681	0	(K)MPAFHPK(Q)
1019.5000	1019.4752	100.0	24.3	739	747	1	(R)STRDDPSNK(E)
1019.5000	1019.5203	100.0	-20.0	293	301	1	(K)NHMKAHGPK(T)
1019.5000	1019.5408	100.0	-40.0	427	435	0	(K)VAEPAEYLK(Y)
1094.5900	1094.5728	100.0	15.7	455	463	0	(R)EYVLVSQEK(R)
1094.5900	1094.6033	100.0	-12.2	285	292	1	(R)FKEPWFLK(N)
1113.6500	1113.6262	100.0	21.3	928	938	0	(R)SATPTPTVIAR(A)
1123.5600	1123.5378	100.0	19.7	1044	1053	0	(K)QEPVAEGHEK(R)
1147.5900	1147.5524	100.0	32.7	4	12	1	(R)NREAEMELR(R)
1307.5700	1307.5620	100.0	6.16	64	74	1	(K)CPYCDHRASQK(G)
1474.7400	1474.7860	100.0	-31.2	644	658	0	(K)AGIAASVSILENSSR(E)
1615.7800	1615.7558	100.0	15.0	365	380	1	(R)TRAPAEEGAEGPSDTK(Q)
2071.0000	2071.0852	100.0	-41.1	838	859	1	(R)TQVPGGMPGSKSGSSPLGVVTK(A)
2092.0300	2092.0517	100.0	-10.4	638	658	1	(R)DTGESKAGIAASVSILENSSR(E)
2409.1900	2409.2204	100.0	-12.6	887	909	0	(R)QTKPCHGQEPHGAATQGPLAKPR(Q)
2795.4000	2795.4475	100.0	-17.0	939	967	1	(R)AGAQPSANSKPVEKFGVPPAGAGFAPTNK(H)
2807.4200	2807.3119	100.0	38.5	682	709	0	(K)QEVPVPGDGVEFPSSTGAEGQTGHPAEK(L)

Num Unmatched Masses: 121

Search for disulfide linked peptides.

Do a non-specific cleavage search.

Search for another component.

The matched peptides cover 20.6% (239/1163AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 523380

3. 13/135 matches (9%).
Acc. #: A2BJL3 Species: HYPBU Name: Carbamoyl-phosphate synthase large chain
Index: 47130 MW: 119164 Da pI: 5.3

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
985.5500	985.5538	100.0	-3.81	865	874	1	(R)AAAEAALRGR(I)
1046.5600	1046.5378	100.0	21.2	937	945	0	(K)SWLSVQGNR(I)
1069.5700	1069.6225	100.0	-49.1	518	526	1	(R)RSIGLDRPR(V)
1147.5900	1147.5565	100.0	29.2	908	918	0	(R)GAYPGLGPEMR(S)
1170.6600	1170.6589	100.0	0.900	663	673	1	(R)LLGTPGRSVDR(A)
1312.6600	1312.6783	100.0	-14.0	618	627	0	(K)LYFEELTLER(V)
1379.6700	1379.6842	100.0	-10.3	361	371	1	(R)WDLEKFEGVEK(S)
1410.7200	1410.7410	100.0	-14.9	400	413	0	(R)MLDIGEPGVVAGPR(Y)
1671.8100	1671.7649	100.0	27.0	291	303	0	(R)DSWEYYVIETNPR(M)
1835.8700	1835.8658	100.0	2.31	757	773	0	(K)FLEDAVEAEIDAVGDSR(R)
1892.0100	1892.0124	100.0	-1.25	139	157	0	(K)AGLPVPPSTPATSVEEALR(A)
1907.9600	1907.9960	100.0	-18.9	495	512	0	(R)LGFSDEQVALLTGTTVEK(V)
2279.1900	2279.2196	100.0	-13.0	887	905	1	(K)LLRPTGWWGVKSPQPSWQR(L)

Num Unmatched Masses: 122

Search for disulfide linked peptides.

Do a non-specific cleavage search.

Search for another component.

The matched peptides cover 15.9% (171/1073AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 47130

4. 13/135 matches (9%).
Acc. #: Q67C40 Species: ORYSJ Name: Mitogen-activated protein kinase 7
Index: 228430 MW: 64670 Da pI: 9.1

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Modifications	Start	End	Missed Cleavages	Sequence
931.4800	931.4520	100.0	30.1		467	474	0	(K)SGYSYNLK(S)
931.4800	931.4884	100.0	-8.97		262	269	0	(K)QPVPFSEK(F)
966.4800	966.5189	100.0	-40.3	1Oxidation	77	84	0	(K)HIMLPPSR(R)
1051.5300	1051.4843	100.0	43.4		514	521	0	(R)SSQYYFTR(S)
1106.5700	1106.6251	100.0	-49.8		77	85	1	(K)HIMLPPSRR(D)
1109.5900	1109.5772	100.0	11.5		456	466	0	(R)NPMVPPAAANK(S)
1113.6500	1113.6303	100.0	17.7		270	279	0	(K)FPNVDPLALK(L)
1125.6100	1125.5721	100.0	33.7	1Oxidation	456	466	0	(R)NPMVPPAAANK(S)
1131.6000	1131.5753	100.0	21.8		522	531	1	(R)SAPRTDLTDR(A)
1556.7700	1556.8002	100.0	-19.4		491	503	0	(R)VQYRPAQHLMDAK(V)
2092.0300	2092.0709	100.0	-19.6		291	309	1	(K)DRPTAEEALADPYFKGLAK(V)
2406.1500	2406.1659	100.0	-6.62		107	125	1	(K)ANDDLTKEHHQFFLYQMLR(A)
2527.4200	2527.3014	100.0	46.9		532	557	0	(R)AALQGSMLYGIAPFNGIAAVAGGYSK(V)
2795.4000	2795.4542	100.0	-19.4		223	247	1	(K)NVVHQLDLMTDLLGTPSMDAISRIR(N)

Num Unmatched Masses: 122

Search for disulfide linked peptides.

Do a non-specific cleavage search.

Search for another component.

The matched peptides cover 29.2% (166/569AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 228430

5. 12/135 matches (8%).
Acc. #: Q9SLZ4 Species: PEA Name: Retinoblastoma-related protein 1
Index: 315543 MW: 114477 Da pI: 7.7

m/z Submitted	MH⁺ Matched	Intensity	Delta ppm	Start	End	Missed Cleavages	Sequence
793.4100	793.4059	100.0	5.18	509	515	0	(R)VLATMCR(A)
863.4900	863.4985	100.0	-9.88	574	580	0	(K)VIESFIR(Y)
918.4600	918.4891	100.0	-31.7	453	460	0	(K)DITSDVIR(R)
931.4800	931.4731	100.0	7.40	35	42	0	(K)NELALDEK(S)
985.5500	985.5789	100.0	-29.3	762	769	0	(R)LQLSQQIR(E)
991.5700	991.5472	100.0	23.0	208	215	1	(R)VHAFSRFK(D)
1351.5800	1351.6172	100.0	-27.5	896	908	0	(K)NDGHLAQCPGSPR(I)
2097.9900	2097.9216	100.0	32.6	293	310	0	(K)KPCLASECETENLENFDR(D)
2397.2200	2397.2633	100.0	-18.1	516	536	1	(R)AEAQILGNNLTSLLTNERFHR(C)
2691.3700	2691.3500	100.0	7.42	770	790	1	(R)ENVYCLFQRILNQWTSLFFNR(H)
2748.4100	2748.3232	100.0	31.6	896	921	1	(K)NDGHLAQCPGSPRISPFPSLPDMSPK(K)
2795.4000	2795.4735	100.0	-26.3	791	814	1	(R)HIDQIILCCFYGVAKISQLNLTFR(E)

Num Unmatched Masses: 123

Search for disulfide linked peptides.

Do a non-specific cleavage search.

Search for another component.

The matched peptides cover 15.2% (156/1026AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #): 315543