Exercise MSMS4
Topic
Protein inference
Experimental
Data from an Orbitrap, cysteine unmodified, tryptic digest.
Search human sequences in Swiss-Prot with precursor tolerance of 10ppm and fragment tolerance of 0.6 Da.
Use Oxidation (M) and Deamidated (NQ) as variable modifications.
Don't forget to select a decoy search and include a contaminants database.
Data
Right-click this link:
MSMS4.mgf,
and choose Save to file
Questions
- How many distinct proteins can you be confident are present in this sample?
Hints |
Mascot results |
Answers
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