Database Search Exercises > Exercise MSMS4 > Answers  
 
 

Exercise MSMS4: Answers

 

  1. How many distinct proteins can you be confident are present in this sample?

    Set 1% FDR (preferably for distinct sequences) and in report builder add a filter 'Num. of significant unique sequences' > 1. This leaves a list of 71 proteins.

    Notice how some families are reduced to a single member, e.g. family 12. Do you think one unique peptide is sufficient evidence for HS71A_HUMAN?

    Apply the same criteria to the decoy results and there are no proteins.

 

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