Exercise SQ2
Topic
A trickier sequence tag search
Experimental
A protein mixture (not alkylated) was separated on a 2D gel. A spot was extracted, digested with trypsin,
and analysed on a ESI-QUAD-TOF. A tag has been interpreted from one of the MS/MS spectra.
Precursor m/z is 703.74 with charge 2+. The low mass end of the tag starts at m/z 147.07.
Residue code i means I or L. Search Swiss-Prot with precursor and fragment mass tolerances of 0.5 Da
Tips
- If you cannot get a match with a standard tag, try an error tolerant tag
Questions
- What is the peptide sequence?
- Are there any alternative solutions?
- If so, how do you choose a preferred solution?
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Answers
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