Exercise MSMS7

Topic

BSA Standard containing many strong matches. Use trial and error to determine settings such as mass tolerances. Use an error tolerant search to decide if any variable modifications need to be included. Switch to the Protein Family Summary to get counts of modifications.

Experimental

BSA tryptic digest data from an QTof, cysteine residues reduced and alkylated with iodoacetamide (+57).

Data

Right-click this link: MSMS7.mgf, and choose Save to file

Questions

1. What are suitable search parameters?
2. What advice would you give to the person who prepared the sample?
3. What advice would you give to the mass spectrometer operator?

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