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Exercise MSMS5

Topic

ETD spectra

Experimental

Data from BSA analysed on an Orbitrap using ETD. Cysteine residues reduced and alkylated with iodoacetic acid (+58). Can use precursor tolerance of 2 ppm and fragment tolerance of 0.1 Da. Trypsin with 1 missed cleavage.

Data

Right-click this link: MSMS5.mgf, and choose Save to file

Questions

  1. Did the peak detection ever fail to pick the 12C peak?
  2. What do you get if you search as CID?

 

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