Database Search Exercises > Exercise MSMS1  
 
 

Exercise MSMS1

Topic

Phosphorylation site analysis

Experimental

Search SwissProt with a taxonomy filter of human using Trypsin; 2 missed cleavages; Phosphorylation of Ser, Thr, and Tyr as variable modifications; approx 0.6 Da for peptide tolerance and 0.4 for fragment tolerance; ESI-QUAD-TOF

Data

Right-click this link: MSMS1.mgf, and choose Save to file

Tips

  • If you don't get a match to FKGPGDTSNFDDYEEEEIRVSINEK, this may be because the search engine doesn't allow more than 1 missed cleavage. If so, try using Lys-C

Questions

  1. Can you be sure which residue is phosphorylated in FKGPGDTSNFDDYEEEEIRVSINEK?
  2. How about TWTLCGTPEYLAPEIILSK?

 

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